March 8-10, 2017
September 27-29, 2017
This 3-day workshop is designed for those with little or no experience in molecular biology. We will cover the basics of nucleic acids, restriction enzyme use, DNA electrophoresis, purification of DNA fragments from agarose gels, preparation of plasmid vectors for subcloning and features and uses of plasmid cloning vectors, DNA ligation reactions, transformation of chemically competent E. coli, screening of recombinant clones, the isolation and analysis of recombinant E. coli clones, and finally an overview of cDNA library construction.
The BioSciConcepts workshops are designed to provide a solid understanding of specific topics through classroom presentation and laboratory work. Clients will gain significant experience in the performance of laboratory techniques taught in each workshop. Through integrated learning methods, utilizing hands-on training to reinforce lecture material, clients will be able to apply information learned in BioSciConcepts workshops into applications in their own laboratories. At the end of the workshop we will conduct a full workshop review, and data troubleshooting session.
Contact: Timothy Fawcett, Ph.D., Director